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lc2005 western blotting wash buffer  (Thermo Fisher)


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    Thermo Fisher lc2005 western blotting wash buffer
    Lc2005 Western Blotting Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc2005+western+blotting+wash+buffer/Tween+20/pm41046517-364-105-104
    Average 99 stars, based on 1 article reviews
    lc2005 western blotting wash buffer - by Bioz Stars, 2026-09
    99/100 stars

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    Transfection:

    Article Title: PNPLA3-I148M is a neomorph that interferes with two primary hepatic triglyceride clearance pathways.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APOB EMD 178467, RRID: AB_2057094 BSA Thermo Fisher Scientific A11133; RRID: AB_2534139 CES1 Thermo Fisher Scientific MA5-24244; RRID: AB_2609162 CIDEB Proteintech 27600-1-AP; RRID: AB_2880922 β-COP Thermo Fisher Scientific PA1-061; RRID: AB_2081296 CGI-58 Sigma-Aldrich WH0051099M1; RRID: AB_1839292 ε-COP Atlas Antibodies HPA043576; RRID: AB_2678563 FLAG tag Sigma-Aldrich F1804; RRID: AB_262044 MTTP Thermo Fisher Scientific PA5-42391; RRID: AB_2608504 Nrf1 Cell Signaling Technology 8052; RRID: AB_11178947 PNPLA2 Cell Signaling Technology 2138; RRID: AB_2167955 Poly-ubiquitin Enzo Life Sciences ADI-SPA-200; RRID: AB_10618379 Sec23A Cell Signaling Technology 8162; RRID: AB_10859891 Sec31A Cell Signaling Technology 13466; RRID: AB_2798228 TM6SF2 Thermo Fisher Scientific PA5-69304; RRID: AB_2690158 α-tubulin Cell Signaling Technology 2144; RRID: AB_2210548 Rabbit anti-goat IgG-HRP Bio-Rad 1721034; RRID: AB_11125144 Goat anti-mouse IgG-HRP Bio-Rad 1706516; RRID: AB_2921252 Goat anti-rabbit IgG-HRP Bio-Rad 1706515; RRID: AB_11125142 Bacterial and virus strains ABHD5 (NM_016006) Human Tagged ORF Clone Lentiviral Particle Origene RC201869L3V MTTP (NM_000253) Human Tagged ORF Clone Lentiviral Particle Origene RC213699L1V Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Tagged ORF Clone Lentiviral Particle Origene RC205708L3V Chemicals, peptides, and recombinant proteins Eagle’s Minimum Essential Medium (EMEM) ATCC 30–2003 EMEM without Phenol Red and L-Glutamine Quality Biological 112-212-101 Dulbecco’s Modified Eagle’s Medium (DMEM) Gibco 11965092 Williams’ Medium E Sigma-Aldrich W1878 Dialyzed Fetal Bovine Serum Gibco 26400044 Lipoprotein Deficient Serum from fetal calf Sigma-Aldrich S5394 Antibiotic-Antimycotic (100×) Gibco 15240096 L-Glutamine (200 mM) Gibco 25030081 Dulbecco’s Phosphate Buffered Saline (DPBS), no calcium, no magnesium Gibco 14190136 TrypLE TM Express Enzyme (1×), phenol red Gibco 12605010 Opti-MEM TM I Reduced Serum Medium Gibco 31985062 Insulin-Transferrin-Selenium (ITS -G) (100×) Gibco 41400045 Penicillin-Streptomycin (5,000 U/mL) Gibco 15070063 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008 Trypan Blue, 0.4% Gibco 15250061 (Continued on next page) 18 Cell Reports 44, 116371, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene Sigma-Aldrich TR-1003-G Poloxamer-407 Sigma-Aldrich 16758-250G Puromycin Dihydrochloride Gibco A1113803 Lipofectamine TM RNAiMAX Transfection Reagent Invitrogen 13778150 Lipofectamine TM 3000 Transfection Reagent Invitrogen L3000008 β-mercaptoethanol Millipore-Sigma 444203 NuPAGE TM Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0–1.5 mm Invitrogen EA0375BOX NuPAGE TM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Invitrogen NP0321BOX NuPAGE TM Tris-Acetate SDS Running Buffer (20×) Invitrogen LA0041 NuPAGE TM MES SDS Running Buffer (20×) Invitrogen NP0002 HiMark TM Pre-stained Protein Standard Invitrogen LC5699 Color Prestained Protein Standard, Broad Range (10–250 kDa) New England Biolabs P7719S Invitrolon TM PVDF/Filter Paper Sandwiches, 0.45 μm, 8.3 × 7.3 cm Invitrogen LC2005 Western Blotting Wash Buffer (PBS +0.1% Tween 20) Amgen N/A Pefabloc® SC Roche 11429868001 RIPA Lysis and Extraction Buffer Thermo Scientific 89900 cOmplete TM EDTA-free Protease Inhibitor Cocktail Roche 11836170001 Halt TM Protease Inhibitor Cocktail, EDTA-Free Thermo Scientific 87785 Halt TM Protease-Phosphatase Inhibitor cocktail Thermo Scientific 78440 PhosSTOP TM Phosphatase Inhibitor Roche 4906845001 Triacsin C Cayman Chemical 10007448 16:0-18:0 PC Avanti Research 850456 16:0-20:4 PC Avanti Research 850459 CB-5083 Cayman Chemical 19311 Brefeldin A Sigma-Aldrich B6542 SPLASH® LIPIDOMIX® Mass Spec Standard Avanti Research 330707 Hoechst 33342 Thermo Scientific 62249 HCS LipidTOX TM Green Neutral Lipid Stain Invitrogen H34475 Pierce TM 16% Formaldehyde (w/v), Methanol-free Thermo Scientific 28906 Bafilomycin A1 Sigma-Aldrich 19–148 di-4-ANEPPDHQ Invitrogen D36802 TRIzol TM Reagent Invitrogen 15596026 RQ1 RNase-Free DNase Promega M6101 Isopropyl Alcohol Sigma-Aldrich I9526 Triglyceride standard Pointe Scientific T7531-STD Cholesterol standard Thermo Scientific 23-666-198 Critical commercial assays Human ApoB ELISA Kit Invitrogen EH34RB Mouse ApoB ELISA Kit Abcam ab230932 DC TM Protein Assay Bio-Rad 5000112 RNeasy Mini Kit Qiagen 74104 or 74106 TaqMan TM RNA-to-Ct TM 1-Step Kit Applied Biosystems 4392938 or 4392656 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 Gaussia Luciferase Glow Assay Kit Thermo Scientific 16160 (Continued on next page) Cell Reports 44, 116371, October 28, 2025 19 ..

    Western Blot:

    Article Title: PNPLA3-I148M is a neomorph that interferes with two primary hepatic triglyceride clearance pathways.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APOB EMD 178467, RRID: AB_2057094 BSA Thermo Fisher Scientific A11133; RRID: AB_2534139 CES1 Thermo Fisher Scientific MA5-24244; RRID: AB_2609162 CIDEB Proteintech 27600-1-AP; RRID: AB_2880922 β-COP Thermo Fisher Scientific PA1-061; RRID: AB_2081296 CGI-58 Sigma-Aldrich WH0051099M1; RRID: AB_1839292 ε-COP Atlas Antibodies HPA043576; RRID: AB_2678563 FLAG tag Sigma-Aldrich F1804; RRID: AB_262044 MTTP Thermo Fisher Scientific PA5-42391; RRID: AB_2608504 Nrf1 Cell Signaling Technology 8052; RRID: AB_11178947 PNPLA2 Cell Signaling Technology 2138; RRID: AB_2167955 Poly-ubiquitin Enzo Life Sciences ADI-SPA-200; RRID: AB_10618379 Sec23A Cell Signaling Technology 8162; RRID: AB_10859891 Sec31A Cell Signaling Technology 13466; RRID: AB_2798228 TM6SF2 Thermo Fisher Scientific PA5-69304; RRID: AB_2690158 α-tubulin Cell Signaling Technology 2144; RRID: AB_2210548 Rabbit anti-goat IgG-HRP Bio-Rad 1721034; RRID: AB_11125144 Goat anti-mouse IgG-HRP Bio-Rad 1706516; RRID: AB_2921252 Goat anti-rabbit IgG-HRP Bio-Rad 1706515; RRID: AB_11125142 Bacterial and virus strains ABHD5 (NM_016006) Human Tagged ORF Clone Lentiviral Particle Origene RC201869L3V MTTP (NM_000253) Human Tagged ORF Clone Lentiviral Particle Origene RC213699L1V Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Tagged ORF Clone Lentiviral Particle Origene RC205708L3V Chemicals, peptides, and recombinant proteins Eagle’s Minimum Essential Medium (EMEM) ATCC 30–2003 EMEM without Phenol Red and L-Glutamine Quality Biological 112-212-101 Dulbecco’s Modified Eagle’s Medium (DMEM) Gibco 11965092 Williams’ Medium E Sigma-Aldrich W1878 Dialyzed Fetal Bovine Serum Gibco 26400044 Lipoprotein Deficient Serum from fetal calf Sigma-Aldrich S5394 Antibiotic-Antimycotic (100×) Gibco 15240096 L-Glutamine (200 mM) Gibco 25030081 Dulbecco’s Phosphate Buffered Saline (DPBS), no calcium, no magnesium Gibco 14190136 TrypLE TM Express Enzyme (1×), phenol red Gibco 12605010 Opti-MEM TM I Reduced Serum Medium Gibco 31985062 Insulin-Transferrin-Selenium (ITS -G) (100×) Gibco 41400045 Penicillin-Streptomycin (5,000 U/mL) Gibco 15070063 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008 Trypan Blue, 0.4% Gibco 15250061 (Continued on next page) 18 Cell Reports 44, 116371, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene Sigma-Aldrich TR-1003-G Poloxamer-407 Sigma-Aldrich 16758-250G Puromycin Dihydrochloride Gibco A1113803 Lipofectamine TM RNAiMAX Transfection Reagent Invitrogen 13778150 Lipofectamine TM 3000 Transfection Reagent Invitrogen L3000008 β-mercaptoethanol Millipore-Sigma 444203 NuPAGE TM Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0–1.5 mm Invitrogen EA0375BOX NuPAGE TM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Invitrogen NP0321BOX NuPAGE TM Tris-Acetate SDS Running Buffer (20×) Invitrogen LA0041 NuPAGE TM MES SDS Running Buffer (20×) Invitrogen NP0002 HiMark TM Pre-stained Protein Standard Invitrogen LC5699 Color Prestained Protein Standard, Broad Range (10–250 kDa) New England Biolabs P7719S Invitrolon TM PVDF/Filter Paper Sandwiches, 0.45 μm, 8.3 × 7.3 cm Invitrogen LC2005 Western Blotting Wash Buffer (PBS +0.1% Tween 20) Amgen N/A Pefabloc® SC Roche 11429868001 RIPA Lysis and Extraction Buffer Thermo Scientific 89900 cOmplete TM EDTA-free Protease Inhibitor Cocktail Roche 11836170001 Halt TM Protease Inhibitor Cocktail, EDTA-Free Thermo Scientific 87785 Halt TM Protease-Phosphatase Inhibitor cocktail Thermo Scientific 78440 PhosSTOP TM Phosphatase Inhibitor Roche 4906845001 Triacsin C Cayman Chemical 10007448 16:0-18:0 PC Avanti Research 850456 16:0-20:4 PC Avanti Research 850459 CB-5083 Cayman Chemical 19311 Brefeldin A Sigma-Aldrich B6542 SPLASH® LIPIDOMIX® Mass Spec Standard Avanti Research 330707 Hoechst 33342 Thermo Scientific 62249 HCS LipidTOX TM Green Neutral Lipid Stain Invitrogen H34475 Pierce TM 16% Formaldehyde (w/v), Methanol-free Thermo Scientific 28906 Bafilomycin A1 Sigma-Aldrich 19–148 di-4-ANEPPDHQ Invitrogen D36802 TRIzol TM Reagent Invitrogen 15596026 RQ1 RNase-Free DNase Promega M6101 Isopropyl Alcohol Sigma-Aldrich I9526 Triglyceride standard Pointe Scientific T7531-STD Cholesterol standard Thermo Scientific 23-666-198 Critical commercial assays Human ApoB ELISA Kit Invitrogen EH34RB Mouse ApoB ELISA Kit Abcam ab230932 DC TM Protein Assay Bio-Rad 5000112 RNeasy Mini Kit Qiagen 74104 or 74106 TaqMan TM RNA-to-Ct TM 1-Step Kit Applied Biosystems 4392938 or 4392656 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 Gaussia Luciferase Glow Assay Kit Thermo Scientific 16160 (Continued on next page) Cell Reports 44, 116371, October 28, 2025 19 ..

    Lysis:

    Article Title: PNPLA3-I148M is a neomorph that interferes with two primary hepatic triglyceride clearance pathways.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APOB EMD 178467, RRID: AB_2057094 BSA Thermo Fisher Scientific A11133; RRID: AB_2534139 CES1 Thermo Fisher Scientific MA5-24244; RRID: AB_2609162 CIDEB Proteintech 27600-1-AP; RRID: AB_2880922 β-COP Thermo Fisher Scientific PA1-061; RRID: AB_2081296 CGI-58 Sigma-Aldrich WH0051099M1; RRID: AB_1839292 ε-COP Atlas Antibodies HPA043576; RRID: AB_2678563 FLAG tag Sigma-Aldrich F1804; RRID: AB_262044 MTTP Thermo Fisher Scientific PA5-42391; RRID: AB_2608504 Nrf1 Cell Signaling Technology 8052; RRID: AB_11178947 PNPLA2 Cell Signaling Technology 2138; RRID: AB_2167955 Poly-ubiquitin Enzo Life Sciences ADI-SPA-200; RRID: AB_10618379 Sec23A Cell Signaling Technology 8162; RRID: AB_10859891 Sec31A Cell Signaling Technology 13466; RRID: AB_2798228 TM6SF2 Thermo Fisher Scientific PA5-69304; RRID: AB_2690158 α-tubulin Cell Signaling Technology 2144; RRID: AB_2210548 Rabbit anti-goat IgG-HRP Bio-Rad 1721034; RRID: AB_11125144 Goat anti-mouse IgG-HRP Bio-Rad 1706516; RRID: AB_2921252 Goat anti-rabbit IgG-HRP Bio-Rad 1706515; RRID: AB_11125142 Bacterial and virus strains ABHD5 (NM_016006) Human Tagged ORF Clone Lentiviral Particle Origene RC201869L3V MTTP (NM_000253) Human Tagged ORF Clone Lentiviral Particle Origene RC213699L1V Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Tagged ORF Clone Lentiviral Particle Origene RC205708L3V Chemicals, peptides, and recombinant proteins Eagle’s Minimum Essential Medium (EMEM) ATCC 30–2003 EMEM without Phenol Red and L-Glutamine Quality Biological 112-212-101 Dulbecco’s Modified Eagle’s Medium (DMEM) Gibco 11965092 Williams’ Medium E Sigma-Aldrich W1878 Dialyzed Fetal Bovine Serum Gibco 26400044 Lipoprotein Deficient Serum from fetal calf Sigma-Aldrich S5394 Antibiotic-Antimycotic (100×) Gibco 15240096 L-Glutamine (200 mM) Gibco 25030081 Dulbecco’s Phosphate Buffered Saline (DPBS), no calcium, no magnesium Gibco 14190136 TrypLE TM Express Enzyme (1×), phenol red Gibco 12605010 Opti-MEM TM I Reduced Serum Medium Gibco 31985062 Insulin-Transferrin-Selenium (ITS -G) (100×) Gibco 41400045 Penicillin-Streptomycin (5,000 U/mL) Gibco 15070063 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008 Trypan Blue, 0.4% Gibco 15250061 (Continued on next page) 18 Cell Reports 44, 116371, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene Sigma-Aldrich TR-1003-G Poloxamer-407 Sigma-Aldrich 16758-250G Puromycin Dihydrochloride Gibco A1113803 Lipofectamine TM RNAiMAX Transfection Reagent Invitrogen 13778150 Lipofectamine TM 3000 Transfection Reagent Invitrogen L3000008 β-mercaptoethanol Millipore-Sigma 444203 NuPAGE TM Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0–1.5 mm Invitrogen EA0375BOX NuPAGE TM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Invitrogen NP0321BOX NuPAGE TM Tris-Acetate SDS Running Buffer (20×) Invitrogen LA0041 NuPAGE TM MES SDS Running Buffer (20×) Invitrogen NP0002 HiMark TM Pre-stained Protein Standard Invitrogen LC5699 Color Prestained Protein Standard, Broad Range (10–250 kDa) New England Biolabs P7719S Invitrolon TM PVDF/Filter Paper Sandwiches, 0.45 μm, 8.3 × 7.3 cm Invitrogen LC2005 Western Blotting Wash Buffer (PBS +0.1% Tween 20) Amgen N/A Pefabloc® SC Roche 11429868001 RIPA Lysis and Extraction Buffer Thermo Scientific 89900 cOmplete TM EDTA-free Protease Inhibitor Cocktail Roche 11836170001 Halt TM Protease Inhibitor Cocktail, EDTA-Free Thermo Scientific 87785 Halt TM Protease-Phosphatase Inhibitor cocktail Thermo Scientific 78440 PhosSTOP TM Phosphatase Inhibitor Roche 4906845001 Triacsin C Cayman Chemical 10007448 16:0-18:0 PC Avanti Research 850456 16:0-20:4 PC Avanti Research 850459 CB-5083 Cayman Chemical 19311 Brefeldin A Sigma-Aldrich B6542 SPLASH® LIPIDOMIX® Mass Spec Standard Avanti Research 330707 Hoechst 33342 Thermo Scientific 62249 HCS LipidTOX TM Green Neutral Lipid Stain Invitrogen H34475 Pierce TM 16% Formaldehyde (w/v), Methanol-free Thermo Scientific 28906 Bafilomycin A1 Sigma-Aldrich 19–148 di-4-ANEPPDHQ Invitrogen D36802 TRIzol TM Reagent Invitrogen 15596026 RQ1 RNase-Free DNase Promega M6101 Isopropyl Alcohol Sigma-Aldrich I9526 Triglyceride standard Pointe Scientific T7531-STD Cholesterol standard Thermo Scientific 23-666-198 Critical commercial assays Human ApoB ELISA Kit Invitrogen EH34RB Mouse ApoB ELISA Kit Abcam ab230932 DC TM Protein Assay Bio-Rad 5000112 RNeasy Mini Kit Qiagen 74104 or 74106 TaqMan TM RNA-to-Ct TM 1-Step Kit Applied Biosystems 4392938 or 4392656 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 Gaussia Luciferase Glow Assay Kit Thermo Scientific 16160 (Continued on next page) Cell Reports 44, 116371, October 28, 2025 19 ..

    Extraction:

    Article Title: PNPLA3-I148M is a neomorph that interferes with two primary hepatic triglyceride clearance pathways.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APOB EMD 178467, RRID: AB_2057094 BSA Thermo Fisher Scientific A11133; RRID: AB_2534139 CES1 Thermo Fisher Scientific MA5-24244; RRID: AB_2609162 CIDEB Proteintech 27600-1-AP; RRID: AB_2880922 β-COP Thermo Fisher Scientific PA1-061; RRID: AB_2081296 CGI-58 Sigma-Aldrich WH0051099M1; RRID: AB_1839292 ε-COP Atlas Antibodies HPA043576; RRID: AB_2678563 FLAG tag Sigma-Aldrich F1804; RRID: AB_262044 MTTP Thermo Fisher Scientific PA5-42391; RRID: AB_2608504 Nrf1 Cell Signaling Technology 8052; RRID: AB_11178947 PNPLA2 Cell Signaling Technology 2138; RRID: AB_2167955 Poly-ubiquitin Enzo Life Sciences ADI-SPA-200; RRID: AB_10618379 Sec23A Cell Signaling Technology 8162; RRID: AB_10859891 Sec31A Cell Signaling Technology 13466; RRID: AB_2798228 TM6SF2 Thermo Fisher Scientific PA5-69304; RRID: AB_2690158 α-tubulin Cell Signaling Technology 2144; RRID: AB_2210548 Rabbit anti-goat IgG-HRP Bio-Rad 1721034; RRID: AB_11125144 Goat anti-mouse IgG-HRP Bio-Rad 1706516; RRID: AB_2921252 Goat anti-rabbit IgG-HRP Bio-Rad 1706515; RRID: AB_11125142 Bacterial and virus strains ABHD5 (NM_016006) Human Tagged ORF Clone Lentiviral Particle Origene RC201869L3V MTTP (NM_000253) Human Tagged ORF Clone Lentiviral Particle Origene RC213699L1V Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Tagged ORF Clone Lentiviral Particle Origene RC205708L3V Chemicals, peptides, and recombinant proteins Eagle’s Minimum Essential Medium (EMEM) ATCC 30–2003 EMEM without Phenol Red and L-Glutamine Quality Biological 112-212-101 Dulbecco’s Modified Eagle’s Medium (DMEM) Gibco 11965092 Williams’ Medium E Sigma-Aldrich W1878 Dialyzed Fetal Bovine Serum Gibco 26400044 Lipoprotein Deficient Serum from fetal calf Sigma-Aldrich S5394 Antibiotic-Antimycotic (100×) Gibco 15240096 L-Glutamine (200 mM) Gibco 25030081 Dulbecco’s Phosphate Buffered Saline (DPBS), no calcium, no magnesium Gibco 14190136 TrypLE TM Express Enzyme (1×), phenol red Gibco 12605010 Opti-MEM TM I Reduced Serum Medium Gibco 31985062 Insulin-Transferrin-Selenium (ITS -G) (100×) Gibco 41400045 Penicillin-Streptomycin (5,000 U/mL) Gibco 15070063 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008 Trypan Blue, 0.4% Gibco 15250061 (Continued on next page) 18 Cell Reports 44, 116371, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene Sigma-Aldrich TR-1003-G Poloxamer-407 Sigma-Aldrich 16758-250G Puromycin Dihydrochloride Gibco A1113803 Lipofectamine TM RNAiMAX Transfection Reagent Invitrogen 13778150 Lipofectamine TM 3000 Transfection Reagent Invitrogen L3000008 β-mercaptoethanol Millipore-Sigma 444203 NuPAGE TM Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0–1.5 mm Invitrogen EA0375BOX NuPAGE TM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Invitrogen NP0321BOX NuPAGE TM Tris-Acetate SDS Running Buffer (20×) Invitrogen LA0041 NuPAGE TM MES SDS Running Buffer (20×) Invitrogen NP0002 HiMark TM Pre-stained Protein Standard Invitrogen LC5699 Color Prestained Protein Standard, Broad Range (10–250 kDa) New England Biolabs P7719S Invitrolon TM PVDF/Filter Paper Sandwiches, 0.45 μm, 8.3 × 7.3 cm Invitrogen LC2005 Western Blotting Wash Buffer (PBS +0.1% Tween 20) Amgen N/A Pefabloc® SC Roche 11429868001 RIPA Lysis and Extraction Buffer Thermo Scientific 89900 cOmplete TM EDTA-free Protease Inhibitor Cocktail Roche 11836170001 Halt TM Protease Inhibitor Cocktail, EDTA-Free Thermo Scientific 87785 Halt TM Protease-Phosphatase Inhibitor cocktail Thermo Scientific 78440 PhosSTOP TM Phosphatase Inhibitor Roche 4906845001 Triacsin C Cayman Chemical 10007448 16:0-18:0 PC Avanti Research 850456 16:0-20:4 PC Avanti Research 850459 CB-5083 Cayman Chemical 19311 Brefeldin A Sigma-Aldrich B6542 SPLASH® LIPIDOMIX® Mass Spec Standard Avanti Research 330707 Hoechst 33342 Thermo Scientific 62249 HCS LipidTOX TM Green Neutral Lipid Stain Invitrogen H34475 Pierce TM 16% Formaldehyde (w/v), Methanol-free Thermo Scientific 28906 Bafilomycin A1 Sigma-Aldrich 19–148 di-4-ANEPPDHQ Invitrogen D36802 TRIzol TM Reagent Invitrogen 15596026 RQ1 RNase-Free DNase Promega M6101 Isopropyl Alcohol Sigma-Aldrich I9526 Triglyceride standard Pointe Scientific T7531-STD Cholesterol standard Thermo Scientific 23-666-198 Critical commercial assays Human ApoB ELISA Kit Invitrogen EH34RB Mouse ApoB ELISA Kit Abcam ab230932 DC TM Protein Assay Bio-Rad 5000112 RNeasy Mini Kit Qiagen 74104 or 74106 TaqMan TM RNA-to-Ct TM 1-Step Kit Applied Biosystems 4392938 or 4392656 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 Gaussia Luciferase Glow Assay Kit Thermo Scientific 16160 (Continued on next page) Cell Reports 44, 116371, October 28, 2025 19 ..

    Protease Inhibitor:

    Article Title: PNPLA3-I148M is a neomorph that interferes with two primary hepatic triglyceride clearance pathways.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APOB EMD 178467, RRID: AB_2057094 BSA Thermo Fisher Scientific A11133; RRID: AB_2534139 CES1 Thermo Fisher Scientific MA5-24244; RRID: AB_2609162 CIDEB Proteintech 27600-1-AP; RRID: AB_2880922 β-COP Thermo Fisher Scientific PA1-061; RRID: AB_2081296 CGI-58 Sigma-Aldrich WH0051099M1; RRID: AB_1839292 ε-COP Atlas Antibodies HPA043576; RRID: AB_2678563 FLAG tag Sigma-Aldrich F1804; RRID: AB_262044 MTTP Thermo Fisher Scientific PA5-42391; RRID: AB_2608504 Nrf1 Cell Signaling Technology 8052; RRID: AB_11178947 PNPLA2 Cell Signaling Technology 2138; RRID: AB_2167955 Poly-ubiquitin Enzo Life Sciences ADI-SPA-200; RRID: AB_10618379 Sec23A Cell Signaling Technology 8162; RRID: AB_10859891 Sec31A Cell Signaling Technology 13466; RRID: AB_2798228 TM6SF2 Thermo Fisher Scientific PA5-69304; RRID: AB_2690158 α-tubulin Cell Signaling Technology 2144; RRID: AB_2210548 Rabbit anti-goat IgG-HRP Bio-Rad 1721034; RRID: AB_11125144 Goat anti-mouse IgG-HRP Bio-Rad 1706516; RRID: AB_2921252 Goat anti-rabbit IgG-HRP Bio-Rad 1706515; RRID: AB_11125142 Bacterial and virus strains ABHD5 (NM_016006) Human Tagged ORF Clone Lentiviral Particle Origene RC201869L3V MTTP (NM_000253) Human Tagged ORF Clone Lentiviral Particle Origene RC213699L1V Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Tagged ORF Clone Lentiviral Particle Origene RC205708L3V Chemicals, peptides, and recombinant proteins Eagle’s Minimum Essential Medium (EMEM) ATCC 30–2003 EMEM without Phenol Red and L-Glutamine Quality Biological 112-212-101 Dulbecco’s Modified Eagle’s Medium (DMEM) Gibco 11965092 Williams’ Medium E Sigma-Aldrich W1878 Dialyzed Fetal Bovine Serum Gibco 26400044 Lipoprotein Deficient Serum from fetal calf Sigma-Aldrich S5394 Antibiotic-Antimycotic (100×) Gibco 15240096 L-Glutamine (200 mM) Gibco 25030081 Dulbecco’s Phosphate Buffered Saline (DPBS), no calcium, no magnesium Gibco 14190136 TrypLE TM Express Enzyme (1×), phenol red Gibco 12605010 Opti-MEM TM I Reduced Serum Medium Gibco 31985062 Insulin-Transferrin-Selenium (ITS -G) (100×) Gibco 41400045 Penicillin-Streptomycin (5,000 U/mL) Gibco 15070063 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008 Trypan Blue, 0.4% Gibco 15250061 (Continued on next page) 18 Cell Reports 44, 116371, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene Sigma-Aldrich TR-1003-G Poloxamer-407 Sigma-Aldrich 16758-250G Puromycin Dihydrochloride Gibco A1113803 Lipofectamine TM RNAiMAX Transfection Reagent Invitrogen 13778150 Lipofectamine TM 3000 Transfection Reagent Invitrogen L3000008 β-mercaptoethanol Millipore-Sigma 444203 NuPAGE TM Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0–1.5 mm Invitrogen EA0375BOX NuPAGE TM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Invitrogen NP0321BOX NuPAGE TM Tris-Acetate SDS Running Buffer (20×) Invitrogen LA0041 NuPAGE TM MES SDS Running Buffer (20×) Invitrogen NP0002 HiMark TM Pre-stained Protein Standard Invitrogen LC5699 Color Prestained Protein Standard, Broad Range (10–250 kDa) New England Biolabs P7719S Invitrolon TM PVDF/Filter Paper Sandwiches, 0.45 μm, 8.3 × 7.3 cm Invitrogen LC2005 Western Blotting Wash Buffer (PBS +0.1% Tween 20) Amgen N/A Pefabloc® SC Roche 11429868001 RIPA Lysis and Extraction Buffer Thermo Scientific 89900 cOmplete TM EDTA-free Protease Inhibitor Cocktail Roche 11836170001 Halt TM Protease Inhibitor Cocktail, EDTA-Free Thermo Scientific 87785 Halt TM Protease-Phosphatase Inhibitor cocktail Thermo Scientific 78440 PhosSTOP TM Phosphatase Inhibitor Roche 4906845001 Triacsin C Cayman Chemical 10007448 16:0-18:0 PC Avanti Research 850456 16:0-20:4 PC Avanti Research 850459 CB-5083 Cayman Chemical 19311 Brefeldin A Sigma-Aldrich B6542 SPLASH® LIPIDOMIX® Mass Spec Standard Avanti Research 330707 Hoechst 33342 Thermo Scientific 62249 HCS LipidTOX TM Green Neutral Lipid Stain Invitrogen H34475 Pierce TM 16% Formaldehyde (w/v), Methanol-free Thermo Scientific 28906 Bafilomycin A1 Sigma-Aldrich 19–148 di-4-ANEPPDHQ Invitrogen D36802 TRIzol TM Reagent Invitrogen 15596026 RQ1 RNase-Free DNase Promega M6101 Isopropyl Alcohol Sigma-Aldrich I9526 Triglyceride standard Pointe Scientific T7531-STD Cholesterol standard Thermo Scientific 23-666-198 Critical commercial assays Human ApoB ELISA Kit Invitrogen EH34RB Mouse ApoB ELISA Kit Abcam ab230932 DC TM Protein Assay Bio-Rad 5000112 RNeasy Mini Kit Qiagen 74104 or 74106 TaqMan TM RNA-to-Ct TM 1-Step Kit Applied Biosystems 4392938 or 4392656 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 Gaussia Luciferase Glow Assay Kit Thermo Scientific 16160 (Continued on next page) Cell Reports 44, 116371, October 28, 2025 19 ..

    Mass Spectrometry:

    Article Title: PNPLA3-I148M is a neomorph that interferes with two primary hepatic triglyceride clearance pathways.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APOB EMD 178467, RRID: AB_2057094 BSA Thermo Fisher Scientific A11133; RRID: AB_2534139 CES1 Thermo Fisher Scientific MA5-24244; RRID: AB_2609162 CIDEB Proteintech 27600-1-AP; RRID: AB_2880922 β-COP Thermo Fisher Scientific PA1-061; RRID: AB_2081296 CGI-58 Sigma-Aldrich WH0051099M1; RRID: AB_1839292 ε-COP Atlas Antibodies HPA043576; RRID: AB_2678563 FLAG tag Sigma-Aldrich F1804; RRID: AB_262044 MTTP Thermo Fisher Scientific PA5-42391; RRID: AB_2608504 Nrf1 Cell Signaling Technology 8052; RRID: AB_11178947 PNPLA2 Cell Signaling Technology 2138; RRID: AB_2167955 Poly-ubiquitin Enzo Life Sciences ADI-SPA-200; RRID: AB_10618379 Sec23A Cell Signaling Technology 8162; RRID: AB_10859891 Sec31A Cell Signaling Technology 13466; RRID: AB_2798228 TM6SF2 Thermo Fisher Scientific PA5-69304; RRID: AB_2690158 α-tubulin Cell Signaling Technology 2144; RRID: AB_2210548 Rabbit anti-goat IgG-HRP Bio-Rad 1721034; RRID: AB_11125144 Goat anti-mouse IgG-HRP Bio-Rad 1706516; RRID: AB_2921252 Goat anti-rabbit IgG-HRP Bio-Rad 1706515; RRID: AB_11125142 Bacterial and virus strains ABHD5 (NM_016006) Human Tagged ORF Clone Lentiviral Particle Origene RC201869L3V MTTP (NM_000253) Human Tagged ORF Clone Lentiviral Particle Origene RC213699L1V Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Tagged ORF Clone Lentiviral Particle Origene RC205708L3V Chemicals, peptides, and recombinant proteins Eagle’s Minimum Essential Medium (EMEM) ATCC 30–2003 EMEM without Phenol Red and L-Glutamine Quality Biological 112-212-101 Dulbecco’s Modified Eagle’s Medium (DMEM) Gibco 11965092 Williams’ Medium E Sigma-Aldrich W1878 Dialyzed Fetal Bovine Serum Gibco 26400044 Lipoprotein Deficient Serum from fetal calf Sigma-Aldrich S5394 Antibiotic-Antimycotic (100×) Gibco 15240096 L-Glutamine (200 mM) Gibco 25030081 Dulbecco’s Phosphate Buffered Saline (DPBS), no calcium, no magnesium Gibco 14190136 TrypLE TM Express Enzyme (1×), phenol red Gibco 12605010 Opti-MEM TM I Reduced Serum Medium Gibco 31985062 Insulin-Transferrin-Selenium (ITS -G) (100×) Gibco 41400045 Penicillin-Streptomycin (5,000 U/mL) Gibco 15070063 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008 Trypan Blue, 0.4% Gibco 15250061 (Continued on next page) 18 Cell Reports 44, 116371, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene Sigma-Aldrich TR-1003-G Poloxamer-407 Sigma-Aldrich 16758-250G Puromycin Dihydrochloride Gibco A1113803 Lipofectamine TM RNAiMAX Transfection Reagent Invitrogen 13778150 Lipofectamine TM 3000 Transfection Reagent Invitrogen L3000008 β-mercaptoethanol Millipore-Sigma 444203 NuPAGE TM Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0–1.5 mm Invitrogen EA0375BOX NuPAGE TM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Invitrogen NP0321BOX NuPAGE TM Tris-Acetate SDS Running Buffer (20×) Invitrogen LA0041 NuPAGE TM MES SDS Running Buffer (20×) Invitrogen NP0002 HiMark TM Pre-stained Protein Standard Invitrogen LC5699 Color Prestained Protein Standard, Broad Range (10–250 kDa) New England Biolabs P7719S Invitrolon TM PVDF/Filter Paper Sandwiches, 0.45 μm, 8.3 × 7.3 cm Invitrogen LC2005 Western Blotting Wash Buffer (PBS +0.1% Tween 20) Amgen N/A Pefabloc® SC Roche 11429868001 RIPA Lysis and Extraction Buffer Thermo Scientific 89900 cOmplete TM EDTA-free Protease Inhibitor Cocktail Roche 11836170001 Halt TM Protease Inhibitor Cocktail, EDTA-Free Thermo Scientific 87785 Halt TM Protease-Phosphatase Inhibitor cocktail Thermo Scientific 78440 PhosSTOP TM Phosphatase Inhibitor Roche 4906845001 Triacsin C Cayman Chemical 10007448 16:0-18:0 PC Avanti Research 850456 16:0-20:4 PC Avanti Research 850459 CB-5083 Cayman Chemical 19311 Brefeldin A Sigma-Aldrich B6542 SPLASH® LIPIDOMIX® Mass Spec Standard Avanti Research 330707 Hoechst 33342 Thermo Scientific 62249 HCS LipidTOX TM Green Neutral Lipid Stain Invitrogen H34475 Pierce TM 16% Formaldehyde (w/v), Methanol-free Thermo Scientific 28906 Bafilomycin A1 Sigma-Aldrich 19–148 di-4-ANEPPDHQ Invitrogen D36802 TRIzol TM Reagent Invitrogen 15596026 RQ1 RNase-Free DNase Promega M6101 Isopropyl Alcohol Sigma-Aldrich I9526 Triglyceride standard Pointe Scientific T7531-STD Cholesterol standard Thermo Scientific 23-666-198 Critical commercial assays Human ApoB ELISA Kit Invitrogen EH34RB Mouse ApoB ELISA Kit Abcam ab230932 DC TM Protein Assay Bio-Rad 5000112 RNeasy Mini Kit Qiagen 74104 or 74106 TaqMan TM RNA-to-Ct TM 1-Step Kit Applied Biosystems 4392938 or 4392656 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 Gaussia Luciferase Glow Assay Kit Thermo Scientific 16160 (Continued on next page) Cell Reports 44, 116371, October 28, 2025 19 ..

    Staining:

    Article Title: PNPLA3-I148M is a neomorph that interferes with two primary hepatic triglyceride clearance pathways.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APOB EMD 178467, RRID: AB_2057094 BSA Thermo Fisher Scientific A11133; RRID: AB_2534139 CES1 Thermo Fisher Scientific MA5-24244; RRID: AB_2609162 CIDEB Proteintech 27600-1-AP; RRID: AB_2880922 β-COP Thermo Fisher Scientific PA1-061; RRID: AB_2081296 CGI-58 Sigma-Aldrich WH0051099M1; RRID: AB_1839292 ε-COP Atlas Antibodies HPA043576; RRID: AB_2678563 FLAG tag Sigma-Aldrich F1804; RRID: AB_262044 MTTP Thermo Fisher Scientific PA5-42391; RRID: AB_2608504 Nrf1 Cell Signaling Technology 8052; RRID: AB_11178947 PNPLA2 Cell Signaling Technology 2138; RRID: AB_2167955 Poly-ubiquitin Enzo Life Sciences ADI-SPA-200; RRID: AB_10618379 Sec23A Cell Signaling Technology 8162; RRID: AB_10859891 Sec31A Cell Signaling Technology 13466; RRID: AB_2798228 TM6SF2 Thermo Fisher Scientific PA5-69304; RRID: AB_2690158 α-tubulin Cell Signaling Technology 2144; RRID: AB_2210548 Rabbit anti-goat IgG-HRP Bio-Rad 1721034; RRID: AB_11125144 Goat anti-mouse IgG-HRP Bio-Rad 1706516; RRID: AB_2921252 Goat anti-rabbit IgG-HRP Bio-Rad 1706515; RRID: AB_11125142 Bacterial and virus strains ABHD5 (NM_016006) Human Tagged ORF Clone Lentiviral Particle Origene RC201869L3V MTTP (NM_000253) Human Tagged ORF Clone Lentiviral Particle Origene RC213699L1V Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Tagged ORF Clone Lentiviral Particle Origene RC205708L3V Chemicals, peptides, and recombinant proteins Eagle’s Minimum Essential Medium (EMEM) ATCC 30–2003 EMEM without Phenol Red and L-Glutamine Quality Biological 112-212-101 Dulbecco’s Modified Eagle’s Medium (DMEM) Gibco 11965092 Williams’ Medium E Sigma-Aldrich W1878 Dialyzed Fetal Bovine Serum Gibco 26400044 Lipoprotein Deficient Serum from fetal calf Sigma-Aldrich S5394 Antibiotic-Antimycotic (100×) Gibco 15240096 L-Glutamine (200 mM) Gibco 25030081 Dulbecco’s Phosphate Buffered Saline (DPBS), no calcium, no magnesium Gibco 14190136 TrypLE TM Express Enzyme (1×), phenol red Gibco 12605010 Opti-MEM TM I Reduced Serum Medium Gibco 31985062 Insulin-Transferrin-Selenium (ITS -G) (100×) Gibco 41400045 Penicillin-Streptomycin (5,000 U/mL) Gibco 15070063 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008 Trypan Blue, 0.4% Gibco 15250061 (Continued on next page) 18 Cell Reports 44, 116371, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene Sigma-Aldrich TR-1003-G Poloxamer-407 Sigma-Aldrich 16758-250G Puromycin Dihydrochloride Gibco A1113803 Lipofectamine TM RNAiMAX Transfection Reagent Invitrogen 13778150 Lipofectamine TM 3000 Transfection Reagent Invitrogen L3000008 β-mercaptoethanol Millipore-Sigma 444203 NuPAGE TM Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0–1.5 mm Invitrogen EA0375BOX NuPAGE TM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Invitrogen NP0321BOX NuPAGE TM Tris-Acetate SDS Running Buffer (20×) Invitrogen LA0041 NuPAGE TM MES SDS Running Buffer (20×) Invitrogen NP0002 HiMark TM Pre-stained Protein Standard Invitrogen LC5699 Color Prestained Protein Standard, Broad Range (10–250 kDa) New England Biolabs P7719S Invitrolon TM PVDF/Filter Paper Sandwiches, 0.45 μm, 8.3 × 7.3 cm Invitrogen LC2005 Western Blotting Wash Buffer (PBS +0.1% Tween 20) Amgen N/A Pefabloc® SC Roche 11429868001 RIPA Lysis and Extraction Buffer Thermo Scientific 89900 cOmplete TM EDTA-free Protease Inhibitor Cocktail Roche 11836170001 Halt TM Protease Inhibitor Cocktail, EDTA-Free Thermo Scientific 87785 Halt TM Protease-Phosphatase Inhibitor cocktail Thermo Scientific 78440 PhosSTOP TM Phosphatase Inhibitor Roche 4906845001 Triacsin C Cayman Chemical 10007448 16:0-18:0 PC Avanti Research 850456 16:0-20:4 PC Avanti Research 850459 CB-5083 Cayman Chemical 19311 Brefeldin A Sigma-Aldrich B6542 SPLASH® LIPIDOMIX® Mass Spec Standard Avanti Research 330707 Hoechst 33342 Thermo Scientific 62249 HCS LipidTOX TM Green Neutral Lipid Stain Invitrogen H34475 Pierce TM 16% Formaldehyde (w/v), Methanol-free Thermo Scientific 28906 Bafilomycin A1 Sigma-Aldrich 19–148 di-4-ANEPPDHQ Invitrogen D36802 TRIzol TM Reagent Invitrogen 15596026 RQ1 RNase-Free DNase Promega M6101 Isopropyl Alcohol Sigma-Aldrich I9526 Triglyceride standard Pointe Scientific T7531-STD Cholesterol standard Thermo Scientific 23-666-198 Critical commercial assays Human ApoB ELISA Kit Invitrogen EH34RB Mouse ApoB ELISA Kit Abcam ab230932 DC TM Protein Assay Bio-Rad 5000112 RNeasy Mini Kit Qiagen 74104 or 74106 TaqMan TM RNA-to-Ct TM 1-Step Kit Applied Biosystems 4392938 or 4392656 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 Gaussia Luciferase Glow Assay Kit Thermo Scientific 16160 (Continued on next page) Cell Reports 44, 116371, October 28, 2025 19 ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: PNPLA3-I148M is a neomorph that interferes with two primary hepatic triglyceride clearance pathways.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APOB EMD 178467, RRID: AB_2057094 BSA Thermo Fisher Scientific A11133; RRID: AB_2534139 CES1 Thermo Fisher Scientific MA5-24244; RRID: AB_2609162 CIDEB Proteintech 27600-1-AP; RRID: AB_2880922 β-COP Thermo Fisher Scientific PA1-061; RRID: AB_2081296 CGI-58 Sigma-Aldrich WH0051099M1; RRID: AB_1839292 ε-COP Atlas Antibodies HPA043576; RRID: AB_2678563 FLAG tag Sigma-Aldrich F1804; RRID: AB_262044 MTTP Thermo Fisher Scientific PA5-42391; RRID: AB_2608504 Nrf1 Cell Signaling Technology 8052; RRID: AB_11178947 PNPLA2 Cell Signaling Technology 2138; RRID: AB_2167955 Poly-ubiquitin Enzo Life Sciences ADI-SPA-200; RRID: AB_10618379 Sec23A Cell Signaling Technology 8162; RRID: AB_10859891 Sec31A Cell Signaling Technology 13466; RRID: AB_2798228 TM6SF2 Thermo Fisher Scientific PA5-69304; RRID: AB_2690158 α-tubulin Cell Signaling Technology 2144; RRID: AB_2210548 Rabbit anti-goat IgG-HRP Bio-Rad 1721034; RRID: AB_11125144 Goat anti-mouse IgG-HRP Bio-Rad 1706516; RRID: AB_2921252 Goat anti-rabbit IgG-HRP Bio-Rad 1706515; RRID: AB_11125142 Bacterial and virus strains ABHD5 (NM_016006) Human Tagged ORF Clone Lentiviral Particle Origene RC201869L3V MTTP (NM_000253) Human Tagged ORF Clone Lentiviral Particle Origene RC213699L1V Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Tagged ORF Clone Lentiviral Particle Origene RC205708L3V Chemicals, peptides, and recombinant proteins Eagle’s Minimum Essential Medium (EMEM) ATCC 30–2003 EMEM without Phenol Red and L-Glutamine Quality Biological 112-212-101 Dulbecco’s Modified Eagle’s Medium (DMEM) Gibco 11965092 Williams’ Medium E Sigma-Aldrich W1878 Dialyzed Fetal Bovine Serum Gibco 26400044 Lipoprotein Deficient Serum from fetal calf Sigma-Aldrich S5394 Antibiotic-Antimycotic (100×) Gibco 15240096 L-Glutamine (200 mM) Gibco 25030081 Dulbecco’s Phosphate Buffered Saline (DPBS), no calcium, no magnesium Gibco 14190136 TrypLE TM Express Enzyme (1×), phenol red Gibco 12605010 Opti-MEM TM I Reduced Serum Medium Gibco 31985062 Insulin-Transferrin-Selenium (ITS -G) (100×) Gibco 41400045 Penicillin-Streptomycin (5,000 U/mL) Gibco 15070063 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008 Trypan Blue, 0.4% Gibco 15250061 (Continued on next page) 18 Cell Reports 44, 116371, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene Sigma-Aldrich TR-1003-G Poloxamer-407 Sigma-Aldrich 16758-250G Puromycin Dihydrochloride Gibco A1113803 Lipofectamine TM RNAiMAX Transfection Reagent Invitrogen 13778150 Lipofectamine TM 3000 Transfection Reagent Invitrogen L3000008 β-mercaptoethanol Millipore-Sigma 444203 NuPAGE TM Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0–1.5 mm Invitrogen EA0375BOX NuPAGE TM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Invitrogen NP0321BOX NuPAGE TM Tris-Acetate SDS Running Buffer (20×) Invitrogen LA0041 NuPAGE TM MES SDS Running Buffer (20×) Invitrogen NP0002 HiMark TM Pre-stained Protein Standard Invitrogen LC5699 Color Prestained Protein Standard, Broad Range (10–250 kDa) New England Biolabs P7719S Invitrolon TM PVDF/Filter Paper Sandwiches, 0.45 μm, 8.3 × 7.3 cm Invitrogen LC2005 Western Blotting Wash Buffer (PBS +0.1% Tween 20) Amgen N/A Pefabloc® SC Roche 11429868001 RIPA Lysis and Extraction Buffer Thermo Scientific 89900 cOmplete TM EDTA-free Protease Inhibitor Cocktail Roche 11836170001 Halt TM Protease Inhibitor Cocktail, EDTA-Free Thermo Scientific 87785 Halt TM Protease-Phosphatase Inhibitor cocktail Thermo Scientific 78440 PhosSTOP TM Phosphatase Inhibitor Roche 4906845001 Triacsin C Cayman Chemical 10007448 16:0-18:0 PC Avanti Research 850456 16:0-20:4 PC Avanti Research 850459 CB-5083 Cayman Chemical 19311 Brefeldin A Sigma-Aldrich B6542 SPLASH® LIPIDOMIX® Mass Spec Standard Avanti Research 330707 Hoechst 33342 Thermo Scientific 62249 HCS LipidTOX TM Green Neutral Lipid Stain Invitrogen H34475 Pierce TM 16% Formaldehyde (w/v), Methanol-free Thermo Scientific 28906 Bafilomycin A1 Sigma-Aldrich 19–148 di-4-ANEPPDHQ Invitrogen D36802 TRIzol TM Reagent Invitrogen 15596026 RQ1 RNase-Free DNase Promega M6101 Isopropyl Alcohol Sigma-Aldrich I9526 Triglyceride standard Pointe Scientific T7531-STD Cholesterol standard Thermo Scientific 23-666-198 Critical commercial assays Human ApoB ELISA Kit Invitrogen EH34RB Mouse ApoB ELISA Kit Abcam ab230932 DC TM Protein Assay Bio-Rad 5000112 RNeasy Mini Kit Qiagen 74104 or 74106 TaqMan TM RNA-to-Ct TM 1-Step Kit Applied Biosystems 4392938 or 4392656 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 Gaussia Luciferase Glow Assay Kit Thermo Scientific 16160 (Continued on next page) Cell Reports 44, 116371, October 28, 2025 19 ..

    Reverse Transcription:

    Article Title: PNPLA3-I148M is a neomorph that interferes with two primary hepatic triglyceride clearance pathways.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APOB EMD 178467, RRID: AB_2057094 BSA Thermo Fisher Scientific A11133; RRID: AB_2534139 CES1 Thermo Fisher Scientific MA5-24244; RRID: AB_2609162 CIDEB Proteintech 27600-1-AP; RRID: AB_2880922 β-COP Thermo Fisher Scientific PA1-061; RRID: AB_2081296 CGI-58 Sigma-Aldrich WH0051099M1; RRID: AB_1839292 ε-COP Atlas Antibodies HPA043576; RRID: AB_2678563 FLAG tag Sigma-Aldrich F1804; RRID: AB_262044 MTTP Thermo Fisher Scientific PA5-42391; RRID: AB_2608504 Nrf1 Cell Signaling Technology 8052; RRID: AB_11178947 PNPLA2 Cell Signaling Technology 2138; RRID: AB_2167955 Poly-ubiquitin Enzo Life Sciences ADI-SPA-200; RRID: AB_10618379 Sec23A Cell Signaling Technology 8162; RRID: AB_10859891 Sec31A Cell Signaling Technology 13466; RRID: AB_2798228 TM6SF2 Thermo Fisher Scientific PA5-69304; RRID: AB_2690158 α-tubulin Cell Signaling Technology 2144; RRID: AB_2210548 Rabbit anti-goat IgG-HRP Bio-Rad 1721034; RRID: AB_11125144 Goat anti-mouse IgG-HRP Bio-Rad 1706516; RRID: AB_2921252 Goat anti-rabbit IgG-HRP Bio-Rad 1706515; RRID: AB_11125142 Bacterial and virus strains ABHD5 (NM_016006) Human Tagged ORF Clone Lentiviral Particle Origene RC201869L3V MTTP (NM_000253) Human Tagged ORF Clone Lentiviral Particle Origene RC213699L1V Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Tagged ORF Clone Lentiviral Particle Origene RC205708L3V Chemicals, peptides, and recombinant proteins Eagle’s Minimum Essential Medium (EMEM) ATCC 30–2003 EMEM without Phenol Red and L-Glutamine Quality Biological 112-212-101 Dulbecco’s Modified Eagle’s Medium (DMEM) Gibco 11965092 Williams’ Medium E Sigma-Aldrich W1878 Dialyzed Fetal Bovine Serum Gibco 26400044 Lipoprotein Deficient Serum from fetal calf Sigma-Aldrich S5394 Antibiotic-Antimycotic (100×) Gibco 15240096 L-Glutamine (200 mM) Gibco 25030081 Dulbecco’s Phosphate Buffered Saline (DPBS), no calcium, no magnesium Gibco 14190136 TrypLE TM Express Enzyme (1×), phenol red Gibco 12605010 Opti-MEM TM I Reduced Serum Medium Gibco 31985062 Insulin-Transferrin-Selenium (ITS -G) (100×) Gibco 41400045 Penicillin-Streptomycin (5,000 U/mL) Gibco 15070063 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008 Trypan Blue, 0.4% Gibco 15250061 (Continued on next page) 18 Cell Reports 44, 116371, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene Sigma-Aldrich TR-1003-G Poloxamer-407 Sigma-Aldrich 16758-250G Puromycin Dihydrochloride Gibco A1113803 Lipofectamine TM RNAiMAX Transfection Reagent Invitrogen 13778150 Lipofectamine TM 3000 Transfection Reagent Invitrogen L3000008 β-mercaptoethanol Millipore-Sigma 444203 NuPAGE TM Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0–1.5 mm Invitrogen EA0375BOX NuPAGE TM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Invitrogen NP0321BOX NuPAGE TM Tris-Acetate SDS Running Buffer (20×) Invitrogen LA0041 NuPAGE TM MES SDS Running Buffer (20×) Invitrogen NP0002 HiMark TM Pre-stained Protein Standard Invitrogen LC5699 Color Prestained Protein Standard, Broad Range (10–250 kDa) New England Biolabs P7719S Invitrolon TM PVDF/Filter Paper Sandwiches, 0.45 μm, 8.3 × 7.3 cm Invitrogen LC2005 Western Blotting Wash Buffer (PBS +0.1% Tween 20) Amgen N/A Pefabloc® SC Roche 11429868001 RIPA Lysis and Extraction Buffer Thermo Scientific 89900 cOmplete TM EDTA-free Protease Inhibitor Cocktail Roche 11836170001 Halt TM Protease Inhibitor Cocktail, EDTA-Free Thermo Scientific 87785 Halt TM Protease-Phosphatase Inhibitor cocktail Thermo Scientific 78440 PhosSTOP TM Phosphatase Inhibitor Roche 4906845001 Triacsin C Cayman Chemical 10007448 16:0-18:0 PC Avanti Research 850456 16:0-20:4 PC Avanti Research 850459 CB-5083 Cayman Chemical 19311 Brefeldin A Sigma-Aldrich B6542 SPLASH® LIPIDOMIX® Mass Spec Standard Avanti Research 330707 Hoechst 33342 Thermo Scientific 62249 HCS LipidTOX TM Green Neutral Lipid Stain Invitrogen H34475 Pierce TM 16% Formaldehyde (w/v), Methanol-free Thermo Scientific 28906 Bafilomycin A1 Sigma-Aldrich 19–148 di-4-ANEPPDHQ Invitrogen D36802 TRIzol TM Reagent Invitrogen 15596026 RQ1 RNase-Free DNase Promega M6101 Isopropyl Alcohol Sigma-Aldrich I9526 Triglyceride standard Pointe Scientific T7531-STD Cholesterol standard Thermo Scientific 23-666-198 Critical commercial assays Human ApoB ELISA Kit Invitrogen EH34RB Mouse ApoB ELISA Kit Abcam ab230932 DC TM Protein Assay Bio-Rad 5000112 RNeasy Mini Kit Qiagen 74104 or 74106 TaqMan TM RNA-to-Ct TM 1-Step Kit Applied Biosystems 4392938 or 4392656 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 Gaussia Luciferase Glow Assay Kit Thermo Scientific 16160 (Continued on next page) Cell Reports 44, 116371, October 28, 2025 19 ..

    Luciferase:

    Article Title: PNPLA3-I148M is a neomorph that interferes with two primary hepatic triglyceride clearance pathways.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APOB EMD 178467, RRID: AB_2057094 BSA Thermo Fisher Scientific A11133; RRID: AB_2534139 CES1 Thermo Fisher Scientific MA5-24244; RRID: AB_2609162 CIDEB Proteintech 27600-1-AP; RRID: AB_2880922 β-COP Thermo Fisher Scientific PA1-061; RRID: AB_2081296 CGI-58 Sigma-Aldrich WH0051099M1; RRID: AB_1839292 ε-COP Atlas Antibodies HPA043576; RRID: AB_2678563 FLAG tag Sigma-Aldrich F1804; RRID: AB_262044 MTTP Thermo Fisher Scientific PA5-42391; RRID: AB_2608504 Nrf1 Cell Signaling Technology 8052; RRID: AB_11178947 PNPLA2 Cell Signaling Technology 2138; RRID: AB_2167955 Poly-ubiquitin Enzo Life Sciences ADI-SPA-200; RRID: AB_10618379 Sec23A Cell Signaling Technology 8162; RRID: AB_10859891 Sec31A Cell Signaling Technology 13466; RRID: AB_2798228 TM6SF2 Thermo Fisher Scientific PA5-69304; RRID: AB_2690158 α-tubulin Cell Signaling Technology 2144; RRID: AB_2210548 Rabbit anti-goat IgG-HRP Bio-Rad 1721034; RRID: AB_11125144 Goat anti-mouse IgG-HRP Bio-Rad 1706516; RRID: AB_2921252 Goat anti-rabbit IgG-HRP Bio-Rad 1706515; RRID: AB_11125142 Bacterial and virus strains ABHD5 (NM_016006) Human Tagged ORF Clone Lentiviral Particle Origene RC201869L3V MTTP (NM_000253) Human Tagged ORF Clone Lentiviral Particle Origene RC213699L1V Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Tagged ORF Clone Lentiviral Particle Origene RC205708L3V Chemicals, peptides, and recombinant proteins Eagle’s Minimum Essential Medium (EMEM) ATCC 30–2003 EMEM without Phenol Red and L-Glutamine Quality Biological 112-212-101 Dulbecco’s Modified Eagle’s Medium (DMEM) Gibco 11965092 Williams’ Medium E Sigma-Aldrich W1878 Dialyzed Fetal Bovine Serum Gibco 26400044 Lipoprotein Deficient Serum from fetal calf Sigma-Aldrich S5394 Antibiotic-Antimycotic (100×) Gibco 15240096 L-Glutamine (200 mM) Gibco 25030081 Dulbecco’s Phosphate Buffered Saline (DPBS), no calcium, no magnesium Gibco 14190136 TrypLE TM Express Enzyme (1×), phenol red Gibco 12605010 Opti-MEM TM I Reduced Serum Medium Gibco 31985062 Insulin-Transferrin-Selenium (ITS -G) (100×) Gibco 41400045 Penicillin-Streptomycin (5,000 U/mL) Gibco 15070063 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008 Trypan Blue, 0.4% Gibco 15250061 (Continued on next page) 18 Cell Reports 44, 116371, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene Sigma-Aldrich TR-1003-G Poloxamer-407 Sigma-Aldrich 16758-250G Puromycin Dihydrochloride Gibco A1113803 Lipofectamine TM RNAiMAX Transfection Reagent Invitrogen 13778150 Lipofectamine TM 3000 Transfection Reagent Invitrogen L3000008 β-mercaptoethanol Millipore-Sigma 444203 NuPAGE TM Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0–1.5 mm Invitrogen EA0375BOX NuPAGE TM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Invitrogen NP0321BOX NuPAGE TM Tris-Acetate SDS Running Buffer (20×) Invitrogen LA0041 NuPAGE TM MES SDS Running Buffer (20×) Invitrogen NP0002 HiMark TM Pre-stained Protein Standard Invitrogen LC5699 Color Prestained Protein Standard, Broad Range (10–250 kDa) New England Biolabs P7719S Invitrolon TM PVDF/Filter Paper Sandwiches, 0.45 μm, 8.3 × 7.3 cm Invitrogen LC2005 Western Blotting Wash Buffer (PBS +0.1% Tween 20) Amgen N/A Pefabloc® SC Roche 11429868001 RIPA Lysis and Extraction Buffer Thermo Scientific 89900 cOmplete TM EDTA-free Protease Inhibitor Cocktail Roche 11836170001 Halt TM Protease Inhibitor Cocktail, EDTA-Free Thermo Scientific 87785 Halt TM Protease-Phosphatase Inhibitor cocktail Thermo Scientific 78440 PhosSTOP TM Phosphatase Inhibitor Roche 4906845001 Triacsin C Cayman Chemical 10007448 16:0-18:0 PC Avanti Research 850456 16:0-20:4 PC Avanti Research 850459 CB-5083 Cayman Chemical 19311 Brefeldin A Sigma-Aldrich B6542 SPLASH® LIPIDOMIX® Mass Spec Standard Avanti Research 330707 Hoechst 33342 Thermo Scientific 62249 HCS LipidTOX TM Green Neutral Lipid Stain Invitrogen H34475 Pierce TM 16% Formaldehyde (w/v), Methanol-free Thermo Scientific 28906 Bafilomycin A1 Sigma-Aldrich 19–148 di-4-ANEPPDHQ Invitrogen D36802 TRIzol TM Reagent Invitrogen 15596026 RQ1 RNase-Free DNase Promega M6101 Isopropyl Alcohol Sigma-Aldrich I9526 Triglyceride standard Pointe Scientific T7531-STD Cholesterol standard Thermo Scientific 23-666-198 Critical commercial assays Human ApoB ELISA Kit Invitrogen EH34RB Mouse ApoB ELISA Kit Abcam ab230932 DC TM Protein Assay Bio-Rad 5000112 RNeasy Mini Kit Qiagen 74104 or 74106 TaqMan TM RNA-to-Ct TM 1-Step Kit Applied Biosystems 4392938 or 4392656 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 Gaussia Luciferase Glow Assay Kit Thermo Scientific 16160 (Continued on next page) Cell Reports 44, 116371, October 28, 2025 19 ..



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